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Tissue-Tek® OCT embedding compound

Tissue-Tek® OCT cryo embedding compound (compound 4583) is a water soluble blend of PVA, glycols and resins that provides a stable matrix around specimens for cryostat tissue sectioning at temperatures of -10C and below. Tissue-Tek is a gel at room temperature but is solidified at -10°C and below. It can be easily removed with water and/or alcohol at room temperatures. Although originally developed and optimised for cryostat sectioning in pathology laboratories, Tissue-Tek OCT embedding compound  is also an excellent mounting medium for use with cryo-SEM, cryo-FIB/SEM and other cold stages (e.g. from Quorum, Leica, Gatan). Tissue-Tek OCT compound is a non-conductive material by nature, but conductivity can be introduced by mixing it with carbon powder or colloidal graphite for cryo-SEM applications.


Tissue-Tek OCT embedding compound:

  • Water soluble blend of glycols and resins give a stable embedding matrix
  • Superior section quality at cryostat temperatures from -10°C to -40°C
  • Highly soluble in water, alcohol and formalin
  • Good sectioning properties with disposable cryostat blades and solid knives
  • Does not leave residues on slides during staining procedures, eliminating undesirable background staining
  • Tissue, mounted in OCT compound can be frozen between -10°C and -80°C
  • An ideal mounting medium for cryo-SEM, cryo-FIB/SEM and other cold stages

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