High pressure freezer
HPM Live μ developed and manufactured by CryoCapCell is an advanced, automated high pressure freezing system for the vitrification of specimens. HPM Live μ can be used for the vitrification of a wide range of cell and tissue types – a range of holders enables selection of the optimal holder for each specimen type. HPM Live μ is the only existing commercial solution to enable live correlative light and electron microscopy (CLEM) experiments.
- Automatic specimen transfer in 1.26 seconds
- Start-up cycle fully controlled by HPM Live μ
- Preparation cycle lasts 20 minutes
- User defined parameters
- 15” inch HD colour touchscreen
- Data and curves are automatically saved and can be sent by e-mail
- Vitrification of two successive samples in 45 seconds
- Optimized workflow for CryoCapsule®
- Open system and flexible to use – also compatible with a range of sample carriers
- Remote control and online diagnostics if connected to internet
Rapid transfer in 1.26 seconds
CLEM by high pressure freezing enables researchers to go beyond human speed and relate a live cellular event to its ultrastructure. HPM Live μ is a unique tool that optimises the entire workflow of CLEM utilising vitrification by high pressure freezing. At the heart of the process is the CryoCapsule which comprises a special sapphire disc printed with carbon coordinates and a gold spacer maintained together by a polyethylene ring. The CryoCapsule is entirely harmless to biological material and protects the specimen against mechanical damage during specimen preparation.
HPM Live µ is the first high pressure freezing system that can be interfaced directly to a confocal / fluorescence / light microscope to allow the specimen to be examined live before initiating the vitrification process.
HPM Live µ integrates an automated specimen arm with a light microscope by holding the CryoCapsule containing the specimen above the objective. At the appropriate time the arm is activated and in less than two seconds the specimen is vitrified.
Specimen holders and clamps
HPM Live u offers a choice of specimen holders to suit most applications. In addition to CryoCapsule the HPM Live u can be used with standard specimen discs (3mm and 6mm), sapphire discs and copper tubes.
Each specimen holder uses a dedicated clamp to ensure optimal positioning for the vitrification process.
To configure a working HPM Live u you will require the following:
- HPM Live u main instrument
- Specimen clamps of your choice
- Specimen holders to fit clamps
- Liquid Nitrogen dewar (if required)
- Additional accessories
Please add your required items using the quote buttons below and we will endeavour to provide your quotation as quickly as possible
Specification of High Pressure Freezer Live μ
- Width: 995,5mm
- Depth: 1064mm
- Height: 1855mm
- Weight: 450kg
- Voltage: 240V
- Installed power: 7000W
- Dry air gas at 6 bars/87psi
- LN2 dewar capacity: 9 litres
Instrument connected to a nitrogen container of 60 to 100 litres
- LN2 consumption: 25 litres per day, including cooling
- Alcohol reservoir: 0.5 litre
Ordering information:
Main instrument
To configure a working HPM Live u system please add the main instrument and specimen holders to your quote and then add any other accessories from the list of options below:
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HPM Live – Automated High Pressure Freezer
HPM Live is an automated high pressure machine for vitrification in 1.26 second, with remote control and online diagnostic (if connected to internet)• Automatic transfer in 1-26 second• Start-up cycle fully controlled by HPM Live• 20 minute preparation cycle• Each user has his own preset parameters• 15” inch HD colour touchscreen • Data and curves are automatically saved andsent by e-mail• Vitrification of two successive samples in 45 seconds• Optimised for CryoCapsules®• Open system – flexible to use: also compatible with the various sample carriers (carriers of 3, 4 or 6mm tubes etc. existing in the market• The only existing commercial solution for live CLEM (Correlative Light and Electron Microscopy)• Live samples imaging up to 10 minutes• 1 clamp 3mm diameter• 1 clamp for CryoCapsules®• 1 kit of spare parts (03 heated collars, thermocouple sensor, 10 seals)• Station for unloading sample
Price On Request £0.00 Add to basket
HPM Live – Automated High Pressure Freezer
HPM Live is an automated high pressure machine for vitrification in 1.26 second, with remote control and online diagnostic (if connected to internet)• Automatic transfer in 1-26 second• Start-up cycle fully controlled by HPM Live• 20 minute preparation cycle• Each user has his own preset parameters• 15” inch HD colour touchscreen • Data and curves are automatically saved andsent by e-mail• Vitrification of two successive samples in 45 seconds• Optimised for CryoCapsules®• Open system – flexible to use: also compatible with the various sample carriers (carriers of 3, 4 or 6mm tubes etc. existing in the market• The only existing commercial solution for live CLEM (Correlative Light and Electron Microscopy)• Live samples imaging up to 10 minutes• 1 clamp 3mm diameter• 1 clamp for CryoCapsules®• 1 kit of spare parts (03 heated collars, thermocouple sensor, 10 seals)• Station for unloading sample
£0.00Price On Request
Specimen clamps
Please select specimen clamps from the options below and then select the corresponding specimen holders:
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Clamp CC
Specially designed for the exclusive CryoCapsule.
Price On Request £0.00 Add to basket
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Clamp C3
Specially designed for the Ø3mm carriers.
Price On Request £0.00 Add to basket
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Clamp C6
Specially designed for the Ø6mm carriers.
Price On Request £0.00 Add to basket
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Clamp CT
Specially designed for the tubes.
Price On Request £0.00 Add to basket
Specimen carriers
Please select the appropriate carriers for your clamps selected from above:
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CryoCapsule ©
This exclusive tool is specially developed for Live imaging before freezing.
Price On Request £0.00 Add to basket
CryoCapsule ©
This exclusive tool is specially developed for Live imaging before freezing.
£0.00Price On Request -
Carrier 3A
Ø 3 Specimen Carrier (Type A) Cavity 0.1 / 0.2 mm (x50)
Price On Request £0.00 Add to basket
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Carrier 3B
Ø 3 Specimen Carrier (Type B) Cavity 0.3 mm (x50)
Price On Request £0.00 Add to basket
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Carrier 6A
Ø 6 Specimen Carrier (Type A) Cavity 0.1 / 0.2 mm (x50)
Price On Request £0.00 Add to basket
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Carrier 6B
Ø 6 Specimen Carrier (Type B) Cavity 0.3 mm (x50)
Price On Request £0.00 Add to basket
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Copper tube 0.6
Copper tube Ø 0.6 / 0.3 mm x 9 mm (x20)
Price On Request £0.00 Add to basket
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Copper tube 0.5
Copper tube Ø 0.5 / 0.35 mm x 9 mm (x20)
Price On Request £0.00 Add to basket
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Sapphire disc 3
Sapphire disc Ø 3 x0.05mm (x20)
Price On Request £0.00 Add to basket
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Sapphire disc 6
Sapphire disc Ø 6 x0.10mm (x20)
Price On Request £0.00 Add to basket
HPM liquid nitrogen dewars
A liquid nitrogen supply dewar is required to run the HPM Live u. If you do not have an existing dewar please select on of the options below:
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Liquid Nitrogen dewar 60l
Useful capacity (l): 58 -Total capacity (l): 60- Total Height (mm): 915- Inner height (mm): 698- Outer diameter (mm)460- Empty weight (kg): 38- Full weight (kg): 86.5- Static consumption (%/day): 1.8- Max. working pressure (bar) : 1.5- Roller base- insulated flexible hose
Price On Request £0.00 Add to basket
Liquid Nitrogen dewar 60l
Useful capacity (l): 58 -Total capacity (l): 60- Total Height (mm): 915- Inner height (mm): 698- Outer diameter (mm)460- Empty weight (kg): 38- Full weight (kg): 86.5- Static consumption (%/day): 1.8- Max. working pressure (bar) : 1.5- Roller base- insulated flexible hose
£0.00Price On Request -
Liquid Nitrogen dewar 120l
Useful capacity (l): 120- Total capacity (l): 127- Total Height (mm): 1045- Inner height (mm): 853- Outer diameter (mm)570- Empty weight (kg): 62- Full weight (kg): 159- Static consumption (%/day): 1.3- Max. working pressure (bar) : 1.5- Roller base- insulated flexible hose
Price On Request £0.00 Add to basket
Liquid Nitrogen dewar 120l
Useful capacity (l): 120- Total capacity (l): 127- Total Height (mm): 1045- Inner height (mm): 853- Outer diameter (mm)570- Empty weight (kg): 62- Full weight (kg): 159- Static consumption (%/day): 1.3- Max. working pressure (bar) : 1.5- Roller base- insulated flexible hose
£0.00Price On Request -
Liquid Nitrogen level indicator
When an accurate indication of the remaining liquid nitrogen is needed- a digital level indicator can be mounted instead of the float level gauge.
Price On Request £0.00 Add to basket
Liquid Nitrogen level indicator
When an accurate indication of the remaining liquid nitrogen is needed- a digital level indicator can be mounted instead of the float level gauge.
£0.00Price On Request
HPM accessories
For optimal use of the HPM Live u it is sometimes useful to have additional accessories from the options below:
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Loading station
The loading station is a convenient tool to load your sample contained in Cryocapsules- classical carriers from Ø3mm to Ø6mm or tubes. It is furnished without the clamp holder.
Price On Request £0.00 Add to basket
Loading station
The loading station is a convenient tool to load your sample contained in Cryocapsules- classical carriers from Ø3mm to Ø6mm or tubes. It is furnished without the clamp holder.
£0.00Price On Request -
Unloading station
The unloading station allows you to easily unscrew the clamp attached to the clamp holder.
Price On Request £0.00 Add to basket
Unloading station
The unloading station allows you to easily unscrew the clamp attached to the clamp holder.
£0.00Price On Request -
Storage station
The storage station keeps your samples well organized in the liquid nitrogen. It is compatible with cryotubes.
Price On Request £0.00 Add to basket
Storage station
The storage station keeps your samples well organized in the liquid nitrogen. It is compatible with cryotubes.
£0.00Price On Request -
Stereomicroscope
Stereomicroscope Zeiss Stemi 305 EDU, Greenough system with stereoscopic angle of 11 °, zZoom 5:1 (0-8x…4-0x), zoom with clickstops on positions 0-8x-1x-2x-3x4x, free working distance 110 mm, angle of vision with adjustable interocular distance 55…75 mm
Price On Request £0.00 Add to basket
Stereomicroscope
Stereomicroscope Zeiss Stemi 305 EDU, Greenough system with stereoscopic angle of 11 °, zZoom 5:1 (0-8x…4-0x), zoom with clickstops on positions 0-8x-1x-2x-3x4x, free working distance 110 mm, angle of vision with adjustable interocular distance 55…75 mm
£0.00Price On Request -
Small dewar for liquid nitrogen
The clamp holder is collected into this dewar following the vitrification process. Supplementary dewars allow to speed up the vitrification session.
Price On Request £0.00 Add to basket
Small dewar for liquid nitrogen
The clamp holder is collected into this dewar following the vitrification process. Supplementary dewars allow to speed up the vitrification session.
£0.00Price On Request -
Cleaning accessory
Swab for the vitrification chamber cleaning.
Price On Request £0.00 Add to basket
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R221 high contrast embedding resin for CLEM
R221 is a high contrast embedding resin for volume CLEM (100ml bottle). The new R221 resin preserves fluorescence- evacuate charging effects at the surface with only traces of UA. Developed for TEM and SEM applications.
£288.87 Add to basket
R221 high contrast embedding resin for CLEM
R221 is a high contrast embedding resin for volume CLEM (100ml bottle). The new R221 resin preserves fluorescence- evacuate charging effects at the surface with only traces of UA. Developed for TEM and SEM applications.
£288.87