TEM stains

UranyLess is a convenient, ready to use TEM staining substitute for uranyl acetate delivering similar results without the problems with restrictions, toxicity and waste elimination procedures associated with uranyl acetate.

UranyLess is a proprietary mix of multiple lanthanides which comes as a ready to use aqueous solution. It has great affinity for biological material having been tested on a wide range of biological tissue, both animal and plant. UranyLess staining solution is ideal for ultra-fine sections and negative staining. After only one minute of contact, sections or deposits are stained sufficiently to provide good contrast for subsequent TEM imaging. If needed, lead citrate staining  is recommended to enhance contrast – Please see below.

An alternative packaging size of 200ml is for use with the Leica EM Stain automatic stainer.

Lead citrate. Although UranyLess has a strong contasting effect, the use of lead citrate is recommended to enhance the contrast following the classic staining protocol according to Reynolds (1963). We offer a ready to use 3% lead citrate solution in an Airless bottle which is filled without air and delivers lead citrate droplets through a push-pump system, thus eliminating CO2 contamination.