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Lumascope 720

Digital imaging system with automated stage and three colour fluorescence

The Etaluma family of inverted microscopes provide a revolutionary solution to high content imaging analysis. The New Lumascope 720 builds on previous 3-colour capabilities by incorporating an automated XY stage with motorised focus, thus enabling autofocus and z-stacking

Automated XY stage with motorised autofocus and Z-stacks
Walk-away automation with the ability to tile or undertake time-lapse on regions of interest

Versatile and compact design
Built to fit and operate in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations

Solid state optics
Minimises pixel shift and eliminates experimental artefacts

Semrock Brightline® Pinkel filter set with LED light sources
No need to maintain costly and cumbersome arc lamps or mercury bulbs

Optimised for 405 nm, 488 nm, and 594 nm excitation
Ability to image Blue (BFP, DAPI), Green (FITC, Fluo-4, GFP) & Red (mCherry, Texas Red)

Universal file formats
Compatible with multiple analysis programs (e.g. ImageJ/Fiji, Lumaquant, Image-Pro Premier)​

Applications
Cell growth and confluence, cell migration and wound healing, determining transfection efficiency, tracking and quantifying protein expression, following cell differentiation, high content analysis and Intravital studies

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Optics – Blue, Green and red fluorescence; brightfield

Phase Contrast – Phase optional

Objective Options – 2.5x, 4x, 10, 20, 40x, 60 and 100x (oil) objectives

Objective Compatibility – RMS-threaded, infinity corrected, 45 mm parfocal distance

Fluorescence Filters – Blue: excitation 370-410 nm; emission 429-462 nm
Green: excitation 473-491 nm; emission 502-561 nm
Red: excitation 580-598 nm; emission 612-680 nm

Camera – High sensitivity monochrome CMOS sensor; C-mount

Image Formats – JPEG, BMP, TIF, GIF or PNG

Image Size – 100 x 100 to 1900 x 1900 pixels

Field of View – 0.9 x 0.9 mm with a 20x objective

Automated XY Stage – SBS nest, 6- to 1536-well microplates

Stage Travel Range – 110mm x 74 mm

Stage Move Speed – 25mm/sec

Well-to-well Time – Images 96-wells: a)1 coluor focus & image, 13 min b) 3 colour focus and image, 34 min

Computer Requirements – Windows 7, 8, 8.1 & 10

Automation Friendly – SDK available

Power Requirements – USB for Lumascope; 100-240 V, 50-60 Hz for autostage

Dimensions with Phase – 37.4 cm W x 43.8 cm D x 46.8 cm H [14.7 in W x 17.3 in D x 18.4 in H]

Weight – 13 kg (29 Ib) without phase; 14 kg (31 Ib) with phase

Operating Conditions – 4°C-42°C; 5%-95% RH non-condensing

  • Custom design
  • Long-term humidity and temperature resistance
  • Automated XY stage
  • Enables high content imaging
  • 3 Colour Fluorescence
  • Flexible with the ability to overlay
  • Compact footprint

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User Manual

Software

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