Luna FL Automated Fluorescence Cell Counter

Dual fluorescence and brightfield automated cell counter

Dual fluorescence optics and AO/PI nuclear staining make the Logos Biosystems Luna fl automated cell counter the perfect choice for counting primary cells and PBMCs

Nuclear Staining
Dual fluorescence counting using AO (acridine orange) and PI (propidium iodide) ensures accurate viability for even the most challenging cell types (e.g PBMCs). Automatically counts cells excluding debris and non-nucleated cells

Affordable Running Costs
Low cost disposable slides or use the optional Luna reusable slide to further reduce ongoing costs as well as save on plastic waste

Accurate and Reliable Data
Large counting volume of 0.5 microlitres (equivalent to 5 large haemocytometer squares) provides count and viability data you can rely upon

Extensive Cell Information
Histogram with cell size gating and cluster map, provide a clearer picture of your cell population

Easy To Use
Intuitive colour touchscreen interface with no need for special training. Premixed AO/PI staining solution and preset protocol.

Flexible Data Handling
Export count, image or report data for offline viewing or sharing


Instrument Control – Built-in colour touchscreen

Cell Size Range – 1-90 µM, (5-60 µM Optimal)

Cell Concentration Range  – 5 x 10^4 – 1 x 10^7 cells per ml

Counting Time – < 30 seconds (fluorescence)

Dimensions (W × D × H) – 22 × 21 × 9 cm (8.6 × 8.3 × 3.5 in.)

Connectivity – One USB-A port

Data Export Formats – CSV, TIFF and PDF

  • Optional reusable slide
  • Save money and the environment
  • Dual Fluorescence Optics
  • Accurate count and viability data using AO/PI staining
  • Flexible
  • Choice of counting modes
  • Compact standalone design
  • Fits into crowded cell culture labs

Hardware Features

  • Colour touchscreen interface
  • Dual fluorescence and brightfield optics
  • High resolution 5MP camera and optimised LED illumination
  • Precision manual focus control
  • 1 x USB port for data export or connection of an external thermal printer


Software Features

  • Optimise and save protocols for different cell types
  • Digital zoom for a clearer view of your cells
  • Tag function highlights live and dead cells allowing you to assess count accuracy and optimise settings
  • Histogram with cell size gating provides cell size information and allows you to exclude populations of cells based on their size
  • Cluster map shows the proportion of cells in different clump sizes
  • Review data on-board


Data Handling

  • On-board storage of the last 1,000 counts with export via USB drive in CSV format for Excel
  • Save images and reports to USB drive if required
  • Raw and Tagged TIFF images
  • Comprehensive PDF reports with count and viability data, histograms, cluster maps and images
  • Connect an optional thermal printer for hard copies of data for your lab notebook



  • Count mammalian cells stained with AO / PI for accurate viability and count data regardless of cell type or size. Ignore non-nucleated cells (e.g RBCs) or debris
  • Count mammalian cells stained with trypan blue or erythrosin b for viability data
  • Count unstained cells for total cell count data
  • Count beads, insect cells and micro-algae
  • Count yeast cells stained with FDA / PI
  • Quantify GFP transfection efficiency in mammalian cells